Journal article

Non-antibiotic antimicrobial triclosan induces multiple antibiotic resistance through genetic mutation

Ji Lu, Min Jin, Hoang Nguyen Son, Likai Mao, Jie Li, Lachlan JM Coin, Zhiguo Yuan, Jianhua Guo



Antibiotic resistance poses a major threat to public health. Overuse and misuse of antibiotics are generally recognized as the key factors contributing to antibiotic resistance. However, whether non-antibiotic, anti-microbial (NAAM) chemicals can directly induce antibiotic resistance is unclear. We aim to investigate whether the exposure to a NAAM chemical triclosan (TCS) has an impact on inducing antibiotic resistance on Escherichia coli. Here, we report that at a concentration of 0.2 mg/L TCS induces multi-drug resistance in wild-type Escherichia coli after 30-day TCS exposure. The oxidative stress induced by TCS caused genetic mutations in genes such as fabI, frdD, marR, acrR and soxR, an..

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University of Melbourne Researchers


Awarded by Australian Research Council

Funding Acknowledgements

We acknowledge the Australian Research Council for funding support through Future Fellowship (FT170100196). Jianhua Guo would like to thank the support by UQ Foundation Research Excellence Awards. We are grateful to Dr. Eloise Larsen for reviewing and editing the manuscript. We thank Dr. Michael Nefedov of The University of Queensland for assistance with the BD FACSAria (TM) II flow cytometer and data analysis. This work was performed in part at the Queensland node of the Australian National Fabrication Facility.