Journal article

Simulating the Dynamics of Targeted Capture Sequencing with CapSim

Minh Duc Cao, Devika Ganesamoorthy, Lachlan JM Coin

Published : 2017


Motivation Targeted sequencing using capture probes has become increasingly popular in clinical applications due to its scalability and cost-effectiveness. The approach also allows for higher sequencing coverage of the targeted regions resulting in better analysis statistical power. However, because of the dynamics of the hybridisation process, it is difficult to evaluate the efficiency of the probe design prior to the experiments which are time consuming and costly. Results We developed CapSim, a software package for simulation of targeted sequencing. Given a genome sequence and a set of probes, CapSim simulates the fragmentation, the dynamics of probe hybridisation, and the sequencing of t..

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University of Melbourne Researchers

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