Journal article

Scaffolding and Completing Genome Assemblies in Real-time with Nanopore Sequencing

Minh Duc Cao, Son Hoang Nguyen, Devika Ganesamoorthy, Alysha Elliott, Matthew Cooper, Lachlan JM Coin

Published : 2016

Abstract

Abstract Genome assemblies obtained from short read sequencing technologies are often fragmented into many contigs because of the abundance of repetitive sequences. Long read sequencing technologies allow the generation of reads spanning most repeat sequences, providing the opportunity to complete these genome assemblies. However, substantial amounts of sequence data and computational resources are required to overcome the high per-base error rate inherent to these technologies. Furthermore, most existing methods only assemble the genomes after sequencing has completed which could result in either generation of more sequence data at greater cost than required or a low-quality assembly if ins..

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University of Melbourne Researchers