Journal article
STIM1 is required for remodeling of the endoplasmic reticulum and microtubule cytoskeleton in steering growth cones
M Pavez, AC Thompson, HJ Arnott, CB Mitchell, I D’Atri, EK Don, JK Chilton, EK Scott, JY Lin, KM Young, RJ Gasperini, L Foa
Journal of Neuroscience | SOC NEUROSCIENCE | Published : 2019
Abstract
The spatial and temporal regulation of calcium signaling in neuronal growth cones is essential for axon guidance. In growth cones, the endoplasmic reticulum (ER) is a significant source of calcium signals. However, it is not clear whether the ER is remodeled during motile events to localize calcium signals in steering growth cones. The expression of the ER-calcium sensor, stromal interacting molecule 1 (STIM1) is necessary for growth cone steering toward the calcium-dependent guidance cue BDNF, with STIM1 functioning to sustain calcium signals through store-operated calcium entry. However, STIM1 is also required for growth cone steering away from semaphorin- 3a, a guidance cue that does not ..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by the Australian Government Postgraduate Awards to M.P., A.C.T., H.J.A., and C.B.M.; National Health and Medical Research Council Australia Grant 1024145 to L.F. and R.J.G., Grant 1066025 to K.M.Y., R.J.G., and L.F., and Grant 1165616 to L.F., J.Y.L. and R.J.G.; the University of Tasmania to R.J.G.; the Ian Potter Foundation to L.F.; and the Snow Foundation to E.K.D. We thank Prof. Tim Ryan for generously gifting plasmids; Prof. David Small for the early review of the work; and colleagues from the L.F., K.M.Y., J.Y.L., and Prof. David Small laboratories for valuable comments and suggestions.