Journal article

An integrated analysis of myeloid cells identifies gaps in in vitro models of in vivo biology

Nadia Rajab, Paul Angel, Yidi Deng, Jennifer Gu, Vanta Jameson, Mariola Kurowska-Stolarska, Simon Milling, Simon Milling, Chris Pacheco, Matt Rutar, Andrew Laslett, Kim Anh Lê Cao, Jarny Choi, Christine Wells

Published : 2019

Abstract

Summary The Stemformatics myeloid atlas is an integrated transcriptome atlas of human macrophages and dendritic cells that systematically compares freshly isolated tissue-resident, cultured, and stem-cell derived myeloid cell types. We identified two broad classes of tissue-resident macrophages with lung, gut and tumour-associated macrophages most similar to monocytes. Microglia, Kupffer cells and synovial macrophages shared similar profiles with each other, and with cultured macrophages. Pluripotent stem cell-derived macrophages were not reminiscent of fetal-derived cells. Instead, they were characterized by atypical expression of collagen and a highly efferocytotic phenotype. Likewise, Flt..

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