Journal article

Single-cell transcriptome analysis of CD8 T-cell memory inflation

Andrew Highton, Madeleine Zinser, Lian Ni Lee, Claire Hutchings, Catherine De Lara, Chansavath Phetsouphanh, Chris Willberg, Claire Gordon, Paul Klenerman, Emanuele Marchi

Wellcome Open Research | Published : 2019

Abstract

Background : Persistent viruses such as murine cytomegalovirus (MCMV) and adenovirus-based vaccines induce strong, sustained CD8 + T-cell responses, described as memory “inflation”. These retain functionality, home to peripheral organs and are associated with a distinct transcriptional program. Methods : To further define the nature of the transcriptional mechanisms underpinning memory inflation at different sites we used single-cell RNA sequencing of tetramer-sorted cells from MCMV-infected mice, analyzing transcriptional networks in virus-specific populations in the spleen and gut intra-epithelial lymphocytes (IEL). Results : We provide a transcriptional map of T-cell memory and define a m..

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University of Melbourne Researchers