Journal article

Candidate reference method for determination of vitamin D from dried blood spot samples

Rosita Zakaria, Katrina J Allen, Jennifer J Koplin, Peter Roche, Ronda F Greaves

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | WALTER DE GRUYTER GMBH | Published : 2020

Abstract

Background The current millennium has seen an explosion in vitamin D testing with the overarching aim of requests to clinically stratify patients as replete or deficient in vitamin D. At a population level, dried blood spot (DBS) sampling offers a less invasive and more practical application for assessment of vitamin D status. We have therefore aimed to develop a sensitive and robust DBS vitamin D method that is traceable to serum for use in population-based studies. Methods Blood spots, calibrators and controls were prepared by punching a 3.2 mm DBS from filter paper and placed into a 96-well micro-plate. The DBS disk was eluted with a combination of water-methanol and internal standard (IS..

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