Journal article

SUBTRACTIVE HYBRIDIZATION CLONING OF NOVEL GENES DIFFERENTIALLY EXPRESSED DURING INTESTINAL DEVELOPMENT

D BARILA, C MURGIA, F NOBILI, S GAETANI, G PEROZZI

EUROPEAN JOURNAL OF BIOCHEMISTRY | SPRINGER VERLAG | Published : 1994

Abstract

Intestinal genes whose expression is regulated during development and differentiation were identified and cloned from a rat villi cDNA library using a subtracted cDNA probe. The isolated clones are transcribed in the fully differentiated intestinal epithelium 21 days after birth and absent or poorly expressed in the fetal gut at 15 days of gestation. Two of the DRI (differentially-expressed in rat intestine) genes are novel, while the others encode the microvillar protein ezrin and intracellular carrier proteins for retinol and fatty acids. Expression of the newly isolated DRI27 and DRI42 clones parallels epithelial differentiation during development and it is more pronounced in the distal p..

View full abstract

University of Melbourne Researchers