Journal article

METALLOTHIONEIN GENE IS EXPRESSED IN DEVELOPING RAT INTESTINE AND IS INDUCED BY ZINC BUT NOT BY CORTICOSTEROIDS

E MENGHERI, C MURGIA, F VIGNOLINI, F NOBILI, S GAETANI

JOURNAL OF NUTRITION | OXFORD UNIV PRESS | Published : 1993

Abstract

The expression of metallothionein (MT) mRNA during perinatal development of rat intestine and its induction by zinc and corticosteroids were studied. Pregnant rats from d 17 to 22 of gestation and rats at 2, 4, 13 and 21 d of postnatal life were injected with saline solution (control) or with zinc (10 mg/kg body wt) or corticosteroids (1 mg/kg body wt). After 6 h, tissues were removed for analysis. Northern hybridization of polyA + RNA to 32P-MT-cDNA revealed that MT was expressed already at d 17 of fetal life, increased afterwards (reaching the maximal expression around birth) and decreased soon after until weaning. Metallothionein mRNA was markedly induced by zinc at d 18 of fetal life to ..

View full abstract

University of Melbourne Researchers