The Znt4 mutation in lethal milk mice affects intestinal zinc homeostasis through the expression of other Zn transporters
Chiara Murgia, Isabella Vespignani, Rita Rami, Giuditta Perozzi
GENES AND NUTRITION | BMC | Published : 2006
The lethal milk mouse syndrome is caused by a point mutation in the zinc transporter gene ZnT4 resulting in defective zinc secretion in the milk of homozygous mutant dams. Pups of any genotype fed solely on lm milk die within the first two weeks of neonatal life, displaying zinc deficiency symptoms. Homozygous mutant pups survive when foster nursed by wild type dams and show signs of mild zinc deficiency in adulthood. To further investigate the role of ZnT4 in zinc secretion in the intestinal epithelium, we have studied the expression by real time quantitative PCR of mutant ZnT4 and of other zinc transporters of the Zip and ZnT families, in the jejunum of homozygous lm mice and of the isogen..View full abstract
The Authors wish to thank Piera Rami for invaluable help with animal care. This work was supported in part by the FISR grant "Improvement of lipid and mineral contents of milk to enhance its nutraceutical and safety properties" from the Italian Ministry of Research (MIUR).