Control of leaf blade outgrowth and floral organ development by LEUNIG, ANGUSTIFOLIA3 and WOX transcriptional regulators
Fei Zhang, Hui Wang, Shweta Kalve, Tezera W Wolabu, Jin Nakashima, John F Golz, Million Tadege
NEW PHYTOLOGIST | WILEY | Published : 2019
Plant lateral organ development is a complex process involving both transcriptional activation and repression mechanisms. The WOX transcriptional repressor WOX1/STF, the LEUNIG (LUG) transcriptional corepressor and the ANGUSTIFOLIA3 (AN3) transcriptional coactivator play important roles in leaf blade outgrowth and flower development, but how these factors coordinate their activities remains unclear. Here we report physical and genetic interactions among these key regulators of leaf and flower development. We developed a novel in planta transcriptional activation/repression assay and suggest that LUG could function as a transcriptional coactivator during leaf blade development. MtLUG physical..View full abstract
Awarded by National Science Foundation
We thank Drs Michiel Vandenbussche and Zhongchi Liu for providing mutant or transgenic seeds, Vivian Irish for sharing glasshouse space for Arabidopsis plants, Yuhai Cui for providing gateway compatible Y2H and BiFC plasmids, Detlef Weigel for providing pRS300 plasmid, and Gerrit Beemster and Hamada Abdelgawad for their help with automated image analysis software for measuring cell area and cell number. We also thank the Arabidopsis stock center for providing an3 mutant seeds. This research was supported by the National Science Foundation grant IOS-1354422.