Biosynthesis of Diverse Antimicrobial and Antiproliferative Acyloins in Anaerobic Bacteria.
Sebastian Schieferdecker, Gulimila Shabuer, Anne-Catrin Letzel, Barbara Urbansky, Mie Ishida-Ito, Keishi Ishida, Michael Cyrulies, Hans-Martin Dahse, Sacha Pidot, Christian Hertweck
ACS Chemical Biology | Published : 2019
Metabolic profiling and genome mining revealed that anaerobic bacteria have the potential to produce acyloin natural products. In addition to sattazolin A and B, three new sattazolin congeners and a novel acyloin named clostrocyloin were isolated from three strains of Clostridium beijerinckii, a bacterium used for industrial solvent production. Bioactivity profiling showed that the sattazolin derivatives possess antimicrobial activities against mycobacteria and pseudomonads with only low cytotoxicity. Clostrocyloin was found to be mainly active against fungi. The thiamine diphosphate (ThDP)-dependent sattazolin-producing synthase was identified in silico and characterized both in vivo and in..View full abstract