Journal article

Pyrethroid resistance in the pest mite, Halotydeus destructor: Dominance patterns and a new method for resistance screening

Xuan Cheng, Paul A Umina, Siu Fai Lee, Ary A Hoffmann

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2019

Abstract

An L1024F substitution in the para gene, which encodes a subunit of the voltage-gated sodium channel, has been implicated in pyrethroid resistance in a mite pest, Halotydeus destructor, which attacks rape and other grain crops. A high-resolution melt (HRM) genotyping assay was developed for testing the relative pyrethroid susceptibility of different para genotypes and for high-throughput field screening of resistant alleles. The L1024F mutation was found to be incompletely recessive in phenotypic laboratory bioassays with the pyrethroid pesticide, bifenthrin. While the resistance ratio of heterozygotes (RS) to susceptible homozygotes (SS) was  200,000) survived the recommended field rate of ..

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Grants

Funding Acknowledgements

Thanks to Owain Edwards, Nancy Endersby-Harshman, Vanessa White and Qiong Yang for technical assistance. XC is supported by scholarships from the University of Melbourne (MIFRS, MIRS), the Australian Commonwealth Scientific and Research Organization (CSIRO) and the Taiwan Ministry of Education (GSSA). This research was supported by the Australian Grains Research and Development Corporation.