Extensive transcriptional responses are co-ordinated by microRNAs as revealed by Exon-Intron Split Analysis (EISA)
Katherine A Pillman, Kaitlin G Scheer, Emily Hackett-Jones, Klay Saunders, Andrew G Bert, John Toubia, Holly J Whitfield, Sunil Sapkota, Laura Sourdin, Pham Hoang, Thuc D Le, Joseph Cursons, Melissa J Davis, Philip A Gregory, Gregory J Goodall, Cameron P Bracken
Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2019
Epithelial-mesenchymal transition (EMT) has been a subject of intense scrutiny as it facilitates metastasis and alters drug sensitivity. Although EMT-regulatory roles for numerous miRNAs and transcription factors are known, their functions can be difficult to disentangle, in part due to the difficulty in identifying direct miRNA targets from complex datasets and in deciding how to incorporate 'indirect' miRNA effects that may, or may not, represent biologically relevant information. To better understand how miRNAs exert effects throughout the transcriptome during EMT, we employed Exon-Intron Split Analysis (EISA), a bioinformatic technique that separates transcriptional and post-transcriptio..View full abstract
Awarded by National Health and Medical Research Council
Awarded by NHMRC
National Health and Medical Research Council [GNT1069182, 1164669]; NHMRC Fellowship [Goodall GNT1026191]; Royal Adelaide Hospital Research Fund (Bracken, Florey Fellowship). Funding for open access charge: NHMRC grant funding.