Variation of Gut Mucosal Microbiome with Anti- Saccharomyces cerevisiae Antibody Status in Pediatric Crohn Disease

Abstract

Crohn disease (CD) is a chronic relapsing condition possibly caused by a dysbiotic microbiome. Approximately 30% to 60% of patients with CD have anti-Saccharomyces cerevisiae antibody (ASCA), but any association with gut microbiota is unexplored. We hypothesized that ASCA positivity would predict a signature microbial status and clinical phenotype.Methods:Ileocolonic mucosal biopsies were obtained from children with CD (n=135), and controls without inflammatory bowel disease (n=45). Comparison was made between ASCA status, microbial diversity, and clinical characteristics.Results:ASCA was highly specific but poorly sensitive for the diagnosis of CD. In patients with CD, ASCA positivity was a..