Journal article

Active Micropump-Mixer for Rapid Antiplatelet Drug Screening in Whole Blood

Crispin Szydzik, Rose J Brazilek, Farzan Akbaridoust, Charitha de Silva, Mitchell Moon, Ivan Marusic, Andrew SH Ooi, Harshal H Nandurkar, Justin R Hamilton, Arnan Mitchell, Warwick S Nesbitt

Analytical Chemistry | American Chemical Society | Published : 2019

Abstract

There is a need for scalable automated lab-on-chip systems incorporating precise hemodynamic control that can be applied to high-content screening of new more efficacious antiplatelet therapies. This paper reports on the development and characterization of a novel active micropump-mixer microfluidic to address this need. Using a novel reciprocating elastomeric micropump design, we take advantage of the flexible structural and actuation properties of this framework to manage the hemodynamics for on-chip platelet thrombosis assay on type 1 fibrillar collagen, using whole blood. By characterizing and harnessing the complex three-dimensional hemodynamics of the micropump operation in conjunction..

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Grants

Awarded by National Health and Medical Research Council Development


Funding Acknowledgements

We would like to acknowledge Prof. Huyen Tran, Prof. Philip Thompson, Prof. Robert Andrews, Dr. Leon Chan, Dr. Eric K. W. Poon, Mr. Markus Knoerzer, and Mr. Stephen Cody for helpful discussions, support, and technical advice. We thank the staff and students at the Australian Centre for Blood Diseases for help with blood collection. The authors acknowledge the facilities and technical assistance of Monash Micro Imaging (MMI), Monash University. Devices were fabricated at the Micro Nano Research Facility (MNRF), RMIT University. C.S. is a recipient of a Professor Robert and Josephine Shanks Scholarship. R.J.B. is a recipient of an Australian Research Training Program Scholarship. W.S.N. is a recipient of a RMIT Vice Chancellor's Senior Research Fellowship. This work was supported via Australian Research Council Discovery Project and National Health and Medical Research Council Development (APP1153716) Grants.