Journal article

A repository of ENU mutant mouse lines and their potential for male fertility research

CL Kennedy, AEO O'Connor, LG Sanchez-Partida, MK Holland, CC Goodnow, DM de Kretser, MK O'Bryan

MOLECULAR HUMAN REPRODUCTION | OXFORD UNIV PRESS | Published : 2005

Abstract

Many of the proteins and their encoding genes involved in spermatogenesis are unknown, making the specific diagnosis and treatment of infertility in males difficult and highlighting the importance of identifying new genes that are involved in spermatogenesis. Through genome-wide chemical mutagenesis using N-ethyl-N-nitrosourea (ENU) and a three-generation breeding scheme to isolate recessive mutations, we have identified mouse lines with a range of abnormalities relevant to human male fertility. Abnormal phenotypes included hypospermatogenesis, Sertoli cell-only (SCO) seminiferous tubules, germ-cell arrest and abnormal spermiogenesis and were accompanied, in some, with abnormal serum levels ..

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University of Melbourne Researchers