Genomic investigation of Staphylococcus aureus recovered from Gambian women and newborns following an oral dose of intra-partum azithromycin
Abdoulie Bojang, Sarah L Baines, Liam Donovan, Romain Guerillot, Kerrie Stevens, Charlie Higgs, Christian Bottomley, Ousman Secka, Mark B Schultz, Anders Goncalves da Silva, Torsten Seemann, Timothy P Stinear, Anna Roca, Benjamin P Howden
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | OXFORD UNIV PRESS | Published : 2019
BACKGROUND: Oral azithromycin given during labour reduces carriage of bacteria responsible for neonatal sepsis, including Staphylococcus aureus. However, there is concern that this may promote drug resistance. OBJECTIVES: Here, we combine genomic and epidemiological data on S. aureus isolated from mothers and babies in a randomized intra-partum azithromycin trial (PregnAnZI) to describe bacterial population dynamics and resistance mechanisms. METHODS: Participants from both arms of the trial, who carried S. aureus in day 3 and day 28 samples post-intervention, were included. Sixty-six S. aureus isolates (from 7 mothers and 10 babies) underwent comparative genome analyses and the data were th..View full abstract
Awarded by UK MRC
Awarded by UK Department for International Development (DFID)
Awarded by National Health and Medical Research Council of Australia
This work was jointly supported by the Medical Research Council (MRC) Unit The Gambia PhD fellowship and Doherty Applied Microbial Genomics, Department of Microbiology & Immunology, University of Melbourne at The Peter Doherty Institute for Infection & Immunity. The main trial was jointly funded by the UK MRC and the UK Department for International Development (DFID) under the MRC/DFID Concordat agreement (reference number MR/J010391/1) and is also part of the EDCTP2 programme (where EDCTP stands for European & Developing Countries Clinical Trials Partnership) supported by the European Union. B. P. H. (GNT1105905) and T. P. S. (GNT1008549) are supported by the National Health and Medical Research Council of Australia.