Journal article
Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
AB Ghodke, RT Good, JF Golz, DA Russell, O Edwards, C Robin
Scientific Reports | NATURE PORTFOLIO | Published : 2019
Abstract
Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phenotypic effects. While these studies suggest that RNA interference (RNAi) might be used to suppress aphid populations, none have shown effects sufficient for field control. The current study analyses the efficacy of dsRNA directed against previously reported gene-targets on Green peach aphid (Myzus persicae) strains. No silencing effect was observed when dsRNA was delivered in artificial diet with..
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Awarded by Australia-India Strategic Research Fund
Funding Acknowledgements
We thank Dr. Alexandre Fournier-Level for his advice especially with the Image J macroscript. We thank Crystal Jones for technical assistance with the initial aphid experiments. We are grateful to Paul Umina for his general collegiality and for providing us the aphid strains. We also thank Dr. Govind Gujar, Dr. Vinay Kalia, Meenu Singla, Dr. Sujatha Suni, Dr. Steven Whyard and, Dr. Bernard Carroll for their discussions and visits. This research has been supported by the Australia India Strategic Research Fund, Grand Challenge Scheme, Grant Number GCF010009. We also thank Prof. Ed Newbigin for discussions on endonucleases.