Vaccine-Induced Carbohydrate-Specific Memory B Cells Reactivate During Rodent Malaria Infection
Hayley Joseph, Qiao Ye Tan, Ramin Mazhari, Emily M Eriksson, Louis Schofield
FRONTIERS IN IMMUNOLOGY | FRONTIERS MEDIA SA | Published : 2019
A long-standing challenge in malaria is the limited understanding of B cell immunity, previously hampered by lack of tools to phenotype rare antigen-specific cells. Our aim was to develop a method for identifying carbohydrate-specific B cells within lymphocyte populations and to determine whether a candidate vaccine generated functional memory B cells (MBCs) that reactivated upon challenge with Plasmodium (pRBCs). To this end, a new flow cytometric probe was validated and used to determine the kinetics of B cell activation against the candidate vaccine glycosylphosphatidylinositol conjugated to Keyhole Limpet Haemocyanin (GPI-KLH). Additionally, immunized C57BL/6 mice were rested (10 weeks) ..View full abstract
Awarded by National Health and Medical Research Council
Awarded by Bill and Melinda Gates Foundation
This work was supported by the National Health and Medical Research Council [GNT1052580 to HJ and GNT1093311] and the Bill and Melinda Gates Foundation [OPP1136441].