Journal article
A single-cell transcriptome atlas of the adult human retina
SW Lukowski, CY Lo, AA Sharov, Q Nguyen, L Fang, SSC Hung, L Zhu, T Zhang, U Grünert, T Nguyen, A Senabouth, JS Jabbari, E Welby, JC Sowden, HS Waugh, A Mackey, G Pollock, TD Lamb, PY Wang, AW Hewitt Show all
EMBO Journal | WILEY | Published : 2019
Open access
Abstract
The retina is a specialized neural tissue that senses light and initiates image processing. Although the functional organization of specific retina cells has been well studied, the molecular profile of many cell types remains unclear in humans. To comprehensively profile the human retina, we performed single-cell RNA sequencing on 20,009 cells from three donors and compiled a reference transcriptome atlas. Using unsupervised clustering analysis, we identified 18 transcriptionally distinct cell populations representing all known neural retinal cells: rod photoreceptors, cone photoreceptors, Müller glia, bipolar cells, amacrine cells, retinal ganglion cells, horizontal cells, astrocytes, and m..
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Funding Acknowledgements
This publication is part of the Human Cell Atlas (www.humancellatlas.org/publications) and the ANZ Human Eye Cell Atlas Consortium. The human Muller cell line Moorfields/Institute of Ophthalmology-Muller 1 (MIO-M1) was obtained from the UCL Institute of Ophthalmology, London, UK. The authors want to thank David Mackey and Holly Chinnery for critical discussion and helpful feedback for this study, and Prema Finn for technical assistance for the collection of human donor samples. This work was supported by funding from the Ophthalmic Research Institute of Australia (RW, SL), the University of Melbourne De Brettville Trust (RW), and the Kel and Rosie Day Foundation (RW) and GOSHCC (JCS). The Centre for Eye Research Australia receives operational infrastructure support from the Victorian Government.