Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in Mycoplasma genitalium
Miguel Fernandez-Huerta, Kaveesha Bodiyabadu, Juliana Esperalba, Catriona S Bradshaw, Judit Serra-Pladevall, Suzanne M Garland, Candela Fernandez-Naval, Jorgen S Jensen, Tomas Pumarola, Samantha Ebeyan, Marie Lundgren, Lit Yeen Tan, Mateu Espasa, Gerald L Murray
Journal of Clinical Microbiology | AMER SOC MICROBIOLOGY | Published : 2019
Mycoplasma genitalium causes a common sexually transmitted infection with a marked propensity to develop antimicrobial resistance. As few treatment options exist, this poses significant challenges to clinicians. Recent diagnostic advances have resulted in tests that report the simultaneous detection of M. genitalium and any resistance to macrolides, the first-line treatment. This allows for therapy to be tailored to the individual, thereby optimizing treatment outcomes. However, resistance to fluoroquinolones, the second-line treatment, is increasing in M. genitalium In this study, we describe a new assay, MG+parC (beta), which simultaneously reports the detection of M. genitalium and five p..View full abstract
Awarded by Innovations Connections grant from the Department of Industry, Innovation and Science
This work was supported by an Innovations Connections grant (ICG000220) from the Department of Industry, Innovation and Science, the Australian Federal Government, and by a Victorian Medical Research Acceleration Fund grant from the State Government of Victoria, Australia. SpeeDx Pty Ltd also provided support in the form of salaries for authors (L.Y.T., S.E., and M.L.).