Conjugation of an scFab domain to the oligomeric HIV envelope protein for use in immune targeting
Hannah AD King, Christopher A Gonelli, Kirsteen M Tullett, Mireille H Lahoud, Damian FJ Purcell, Heidi E Drummer, Pantelis Poumbourios, Rob J Center
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2019
A promising strategy for the enhancement of vaccine-mediated immune responses is by directly targeting protein antigens to immune cells. Targeting of antigens to the dendritic cell (DC) molecule Clec9A has been shown to enhance antibody affinity and titers for model antigens, and influenza and enterovirus antigens, and may be advantageous for immunogens that otherwise fail to elicit antibodies with sufficient titers and breadth for broad protection, such as the envelope protein (Env) of HIV. Previously employed targeting strategies often utilize receptor-specific antibodies, however it is impractical to conjugate a bivalent IgG antibody to oligomeric antigens, including HIV Env trimers. Here..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
Awarded by NHMRC
Awarded by Australian NHMRC
The work was supported by an Australian Centre for HIV and Hepatitis Virology (ACH2) Research Grant, awarded to RJC, which was entitled: "Targeting HIV immunogens to immune cells to enhance vaccine efficacy". RJC was also supported by a Project Grant from the Australian National Health and Medical Research Council (NHMRC), grant number 1146082. HADK and CAG were supported by Australian Postgraduate Awards, funded by the Australian Federal Government. MHL and KMT were supported by an NHMRC Project Grant APP1082665. DFJP was supported by the University of Melbourne. HED was supported by Project Grants from the Australian NHMRC, grant numbers 1106581 and 1146082, and a Fellowship from the NHMRC, 1041897. PP was supported by an NHMRC project grant, GNT1125822, and a grant awarded by ACH2. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.