V-ATPase-associated prorenin receptor is upregulated in prostate cancer after PTEN loss.
Amro H Mohammad, Sarah Assadian, Frédéric Couture, Karen J Lefebvre, Wissal El-Assaad, Veronique Barrès, Veronique Ouellet, Pierre-Luc Boulay, Jieyi Yang, Mathieu Latour, Luc Furic, William Muller, Nahum Sonenberg, Anne-Marie Mes-Masson, Fred Saad, Robert Day, Jose G Teodoro
Oncotarget | Published : 2019
Phosphatase and tensin homolog (PTEN) tumor suppressor protein loss is common in prostate cancer (PCa). PTEN loss increases PI3K/Akt signaling, which promotes cell growth and survival. To find secreted biomarkers of PTEN loss, a proteomic screen was used to compare secretomes of cells with and without PTEN expression. We showed that PTEN downregulates Prorenin Receptor (PRR) expression and secretion of soluble Prorenin Receptor (sPRR) in PCa cells and in mouse. PRR is an accessory protein required for assembly of the vacuolar ATPase (V-ATPase) complex. V-ATPase is required for lysosomal acidification, amino acid sensing, efficient mechanistic target of Rapamycin complex 1 (mTORC1) activation..View full abstract