Journal article

Gonocyte transformation in congenital undescended testes: what is the role of inhibin-B in cell death?

Vanessa Wilson, Jorgen Thorup, Erick Clasen-Linde, Dina Cortes, John M Hutson, Ruili Li

Pediatric Surgery International | SPRINGER | Published : 2019

Abstract

PURPOSE: Undescended testes (UDT) are subjected to heat stress, which can disturb gonocyte transformation as well as apoptosis. This study aims to describe the apoptosis pathway occurring during minipuberty of children with unilateral (UDT), and to investigate the role of inhibin-B. METHODS: Testicular biopsies at unilateral orchidopexy of 10 boys (6-9 months old) with normal inhibin-B (n = 5) or low inhibin-B (n = 5) were selected for immunohistochemistry and TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labelling) assay. Testicular tubules were labelled with antibodies against Anti-Müllerian hormone (AMH, Sertoli cell marker), mouse Vasa Homolog (MVH) and placental alkaline ph..

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Grants

Awarded by Australian National Health and Medical Research Council


Funding Acknowledgements

This study was supported by the Australian National Health and Medical Research Council (Grant number APP1144752) and the Victorian Government's Operational Infrastructure Support Programme.