Journal article

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Chen Liu, Michael Malek, Ivan Poon, Lanzhou Jiang, Arif M Siddiquee, Colin JR Sheppard, Ann Roberts, Harry Quiney, Douguo Zhang, Xiaocong Yuan, Jiao Lin, Christian Depeursinge, Pierre Marquet, Shan Shan Kou

Photonics Research | OPTICAL SOC AMER | Published : 2019

Abstract

We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T c..

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Grants

Awarded by Australian Research Council (ARC)


Awarded by National Natural Science Foundation of China (NSFC)


Awarded by Shenzhen Science and Technology Innovation Commission


Awarded by Government of Guangdong Province


Awarded by Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung (SNF)


Funding Acknowledgements

Australian Research Council (ARC) (DE120102352); National Natural Science Foundation of China (NSFC) (61427819); Shenzhen Science and Technology Innovation Commission (KQCS2015032416183980); Government of Guangdong Province (00201505); Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung (SNF) (149652).