Journal article

Dietary intake influences gut microbiota development of healthy Australian children from the age of one to two years

Misa Matsuyama, Mark Morrison, Kim-Anh Le Cao, Solange Pruilh, Peter SW Davies, Clare Wall, Amy Lovell, Rebecca J Hill



Early life nutrition is a vital determinant of an individual's life-long health and also directly influences the ecological and functional development of the gut microbiota. However, there are limited longitudinal studies examining the effect of diet on the gut microbiota development in early childhood. Here, up to seven stool samples were collected from each of 48 healthy children during their second year of life, and microbiota dynamics were assessed using 16S rRNA gene amplicon sequencing. Children's dietary information was also collected during the same period using a validated food frequency questionnaire designed for this age group, over five time points. We observed significant change..

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University of Melbourne Researchers


Awarded by National Health and Medical Research Council (NHMRC) Career Development fellowship

Funding Acknowledgements

M. Matsuyama was supported by the Australian government Research and Training Program scholarship. KALC is funded by National Health and Medical Research Council (NHMRC) Career Development fellowship (GNT1087415). The authors thank members of the Morrison Laboratory at the University of Queensland, Dr. Emily Hoedt and Ms Nida Murtaza for their assistance in phylogenetic analysis and Mr. Jing Jie Teh for the detection and removal of chimeric sequences. The authors thank Dr. Anne Bernard from QFAB Bioinformatics at the University of Queensland for biostatistics support provided through the Child Health Research Centre.