Multi-Compartment Profiling of Bacterial and Host Metabolites Identifies Intestinal Dysbiosis and Its Functional Consequences in the Critically Ill Child.
Anisha Wijeyesekera, Josef Wagner, Marcus De Goffau, Sarah Thurston, Adilson Rodrigues Sabino, Sara Zaher, Deborah White, Jenna Ridout, Mark J Peters, Padmanabhan Ramnarayan, Ricardo G Branco, M Estee Torok, Frederic Valla, Rosan Meyer, Nigel Klein, Gary Frost, Julian Parkhill, Elaine Holmes, Nazima Pathan
Critical Care Medicine | Published : 2019
OBJECTIVES: Adverse physiology and antibiotic exposure devastate the intestinal microbiome in critical illness. Time and cost implications limit the immediate clinical potential of microbial sequencing to identify or treat intestinal dysbiosis. Here, we examined whether metabolic profiling is a feasible method of monitoring intestinal dysbiosis in critically ill children. DESIGN: Prospective multicenter cohort study. SETTING: Three U.K.-based PICUs. PATIENTS: Mechanically ventilated critically ill (n = 60) and age-matched healthy children (n = 55). INTERVENTIONS: Collection of urine and fecal samples in children admitted to the PICU. A single fecal and urine sample was collected in healthy c..View full abstract
Awarded by Wellcome Trust
Awarded by Medical Research Council