Induction of Potent CD8 T Cell Cytotoxicity by Specific Targeting of Antigen to Cross-Presenting Dendritic Cells In Vivo via Murine or Human XCR1
Evelyn Hartung, Martina Becker, Annabell Bachem, Nele Reeg, Anika Jaekel, Andreas Hutloff, Harald Weber, Christoph Weise, Claudia Giesecke, Volker Henn, Stephanie Gurka, Konstantinos Anastassiadis, Hans W Mages, Richard A Kroczek
JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2015
Current subunit vaccines are incapable of inducing Ag-specific CD8(+) T cell cytotoxicity needed for the defense of certain infections and for therapy of neoplastic diseases. In experimental vaccines, cytotoxic responses can be elicited by targeting of Ag into cross-presenting dendritic cells (DC), but almost all available systems use target molecules also expressed on other cells and thus lack the desired specificity. In the present work, we induced CD8(+) T cell cytotoxicity by targeting of Ag to XCR1, a chemokine receptor exclusively expressed on murine and human cross-presenting DC. Targeting of Ag with a mAb or the chemokine ligand XCL1 was highly specific, as determined with XCR1-defic..View full abstract
Awarded by Deutsche Forschungsgemeinschaft
This work was supported by grants of the Wilhelm Sander Foundation, the Fritz Thyssen Foundation, the Validation of Innovation program of the Bundesministerium fur Bildung und Forschung, and in part by Deutsche Forschungsgemeinschaft grants to R.A.K. (Kr 827/16-1 and Kr 827/18-1).