Expression of XCR1 characterizes the Batf3-dependent lineage of dendritic cells capable of antigen cross-presentation
Annabell Bachem, Evelyn Hartung, Steffen Guettler, Ahmed Mora, Xuefei Zhou, Anika Hegemann, Maud Plantinga, Elisa Mazzini, Patrizia Stoitzner, Stephanie Gurka, Volker Henn, Hans W Mages, Richard A Kroczek
FRONTIERS IN IMMUNOLOGY | FRONTIERS MEDIA SA | Published : 2012
Cross-presentation of antigen by dendritic cells (DCs) to CD8(+) T cells is a fundamentally important mechanism in the defense against pathogens and tumors. Due to the lack of an appropriate lineage marker, cross-presenting DCs in the mouse are provisionally classified as "Batf3-IRF-8-Id2-dependent DCs" or as "CD8(+) DCs" in the spleen, and as "CD103(+)CD11b(-) DCs" in the periphery. We have now generated a mAb to XCR1, a chemokine receptor which is specifically expressed on CD8(+) DCs and a subpopulation of double negative DCs in the spleen. Using this antibody, we have determined that only XCR1(+)CD8(+) (around 80% of CD8(+) DCs) and their probable precursors, XCR1(+)CD8(-) DCs, efficientl..View full abstract
Awarded by Deutsche Forschungsgemeinschaft
This work was supported by the Wilhelm Sander-Foundation and the Deutsche Forschungsgemeinschaft (Kr 827/16-1 and TR52). Batf3-deficient mice were kindly provided by Hans-Christian Probst, Mainz University, ICSBP KO mice by Rosel Blasig, Leibniz Institute for Molecular Pharmacology (Berlin), CX3CR1<SUP>GFP</SUP> mice by M. Gunzer (Magdeburg), and Lang-EGFP mice by P. Stoitzner (Innsbruck). Anti-murine Clec9A/DNGR-1 mAbs were kindly provided by M. H. Lahoud and I. Caminschi (Melbourne) and C. Reis e Sousa (London). B16 cells secreting Flt3 ligand were a gift of S. Jung (Rehovot). We thank our colleague M. Becker for her technical support.