Journal article
Expression of XCR1 characterizes the Batf3-dependent lineage of dendritic cells capable of antigen cross-presentation
A Bachem, E Hartung, S Güttler, A Mora, X Zhou, A Hegemann, M Plantinga, E Mazzini, P Stoitzner, S Gurka, V Henn, HW Mages, RA Kroczek
Frontiers in Immunology | FRONTIERS MEDIA SA | Published : 2012
Abstract
Cross-presentation of antigen by dendritic cells (DCs) to CD8+ T cells is a fundamentally important mechanism in the defense against pathogens and tumors. Due to the lack of an appropriate lineage marker, cross-presenting DCs in the mouse are provisionally classified as "Batf3-IRF-8-Id2-dependent DCs" or as "CD8+ DCs" in the spleen, and as "CD103+CD11b- DCs" in the periphery. We have now generated a mAb to XCR1, a chemokine receptor which is specifically expressed on CD8+ DCs and a subpopulation of double negative DCs in the spleen. Using this antibody, we have determined that only XCR1+CD8+ (around 80% of CD8+ DCs) and their probable precursors, XCR1CCD8- DCs, efficiently take up cellular m..
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Awarded by Deutsche Forschungsgemeinschaft
Funding Acknowledgements
This work was supported by the Wilhelm Sander-Foundation and the Deutsche Forschungsgemeinschaft (Kr 827/16-1 and TR52). Batf3-deficient mice were kindly provided by Hans-Christian Probst, Mainz University, ICSBP KO mice by Rosel Blasig, Leibniz Institute for Molecular Pharmacology (Berlin), CX3CR1<SUP>GFP</SUP> mice by M. Gunzer (Magdeburg), and Lang-EGFP mice by P. Stoitzner (Innsbruck). Anti-murine Clec9A/DNGR-1 mAbs were kindly provided by M. H. Lahoud and I. Caminschi (Melbourne) and C. Reis e Sousa (London). B16 cells secreting Flt3 ligand were a gift of S. Jung (Rehovot). We thank our colleague M. Becker for her technical support.