Journal article

Macrophage spatial heterogeneity in gastric cancer defined by multiplex immunohistochemistry

Yu-Kuan Huang, Minyu Wang, Yu Sun, Natasha Di Costanzo, Catherine Mitchell, Adrian Achuthan, John A Hamilton, Rita A Busuttil, Alex Boussioutas

NATURE COMMUNICATIONS | NATURE PUBLISHING GROUP | Published : 2019

Abstract

Tumor-associated macrophages (TAMs), one of the most abundant immune components in gastric cancer (GC), are difficult to characterize due to their heterogeneity. Multiple approaches have been used to elucidate the issue, however, due to the tissue-destructive nature of most of these methods, the spatial distribution of TAMs in situ remains unclear. Here we probe the relationship between tumor context and TAM heterogeneity by multiplex immunohistochemistry of 56 human GC cases. Using distinct expression marker profiles on TAMs, we report seven predominant populations distributed between tumor and non-tumor tissue. TAM population-associated gene signatures reflect their heterogeneity and polar..

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Grants

Funding Acknowledgements

We thank Dr. Paul Beavis and A/Prof. Paul Neeson for helpful discussions for the study and for reviewing the paper. We also thank Prof. David Bowtell for reviewing the paper, Dr. Sharon Pattison for digitalizing clinical information and conducting patient followup, the core facilities at the Peter MacCallum Cancer Centre for supporting data collection and analyses, and all patients and staffs involved in the project. Y.H., M.W. and Y.S. are supported by the Australian Commonwealth Government Research Training Program, the University of Melbourne. Y.H. is supported by the Government Scholarship to Study Abroad (GSSA) issued by Taiwan Ministry of Education. M.W. and Y.S. are supported by the Cancer Therapeutics CRC.