Journal article
Display of malaria transmission-blocking antigens on chimeric duck hepatitis B virus-derived virus-like particles produced in Hansenula polymorpha
D Wetzel, JA Chan, M Suckow, A Barbian, M Weniger, V Jenzelewski, L Reiling, JS Richards, DA Anderson, B Kouskousis, C Palmer, E Hanssen, G Schembecker, J Merz, JG Beeson, M Piontek
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2019
Abstract
Background Malaria caused by Plasmodium falciparum is one of the major threats to human health globally. Despite huge efforts in malaria control and eradication, highly effective vaccines are urgently needed, including vaccines that can block malaria transmission. Chimeric virus-like particles (VLP) have emerged as a promising strategy to develop new malaria vaccine candidates. Methods We developed yeast cell lines and processes for the expression of malaria transmission-blocking vaccine candidates Pfs25 and Pfs230 as VLP and VLP were analyzed for purity, size, protein incorporation rate and expression of malaria antigens. Results In this study, a novel platform for the display of Plasmodium..
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Awarded by National Institutes of Health
Funding Acknowledgements
This work was supported by PATH Malaria Vaccine Initiative; National Health and Medical Research Council of Australia (Senior Research Fellowship and Program Grant to JGB). Burnet Institute is supported by funding from the NHMRC Independent Research Institutes Infrastructure Support Scheme and a Victorian State Government Operational Infrastructure grant. ARTES Biotechnology GmbH provided support in the form of salaries for authors DW, MS, MW, VJ and MP. Juliane Merz is employed by Evonik Technology & Infrastructure GmbH. Evonik Technology & Infrastructure GmbH provided support in the form of salary for author JM, but did not have any additional role in the study design, data collection and analysis, or preparation of the manuscript. Evonik Technology & Infrastructure GmbH expressly agreed on publishing the manuscript. The specific roles of JM are articulated in the 'author contributions' section.