Journal article
Glycolysis is required for LPS-induced activation and adhesion of human CD14 CD16− monocytes
MKS Lee, A Al-Sharea, WA Shihata, CB Veiga, OD Cooney, AJ Fleetwood, MC Flynn, E Claeson, CS Palmer, GI Lancaster, DC Henstridge, JA Hamilton, AJ Murphy
Frontiers in Immunology | FRONTIERS MEDIA SA | Published : 2019
Open access
Abstract
Monocytes in humans consist of 3 subsets; CD14+ CD16− (classical), CD14+ CD16+ (intermediate) and CD14dim CD16+ (non-classical), which exhibit distinct and heterogeneous responses to activation. During acute inflammation CD14+ CD16− monocytes are significantly elevated and migrate to the sites of injury via the adhesion cascade. The field of immunometabolism has begun to elucidate the importance of the engagement of specific metabolic pathways in immune cell function. Yet, little is known about monocyte metabolism and the role of metabolism in mediating monocyte activation and adherence to vessels. Accordingly, we aimed to determine whether manipulating the metabolism of CD14+ CD16− monocyte..
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Awarded by CSL Behring
Funding Acknowledgements
This work was supported by NHMRC grant (APP1142398) to AM, GL, and JH. AM was supported by a Centenary Award from CSL. ML was supported by a postdoctoral fellowship from the National Heart foundation (101951).