Journal article

A novel metabarcoding diagnostic tool to explore protozoan haemoparasite diversity in mammals: a proof-of-concept study using canines from the tropics

Lucas G Huggins, Anson Koehler, Ng-Nguyen Dinh, Stephen Wilcox, Bettina Schunack, Tawin Inpankaew, Rebecca J Traub

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2019

Abstract

Haemoparasites are responsible for some of the most prevalent and debilitating canine illnesses across the globe, whilst also posing a significant zoonotic risk to humankind. Nowhere are the effects of such parasites more pronounced than in developing countries in the tropics where the abundance and diversity of ectoparasites that transmit these pathogens reaches its zenith. Here we describe the use of a novel next-generation sequencing (NGS) metabarcoding based approach to screen for a range of blood-borne apicomplexan and kinetoplastid parasites from populations of temple dogs in Bangkok, Thailand. Our methodology elucidated high rates of Hepatozoon canis and Babesia vogeli infection, whil..

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Grants

Awarded by Australian Research Council


Funding Acknowledgements

The authors are particularly grateful for the bioinformatic processing support provided by Ross Hall (Melbourne Veterinary School) and technical advice from Dr Neil Young (Melbourne Veterinary School). This study was funded by an Australian Research Council Linkage grant LP170100187 with Bayer Animal Health GmbH and Bayer Australia as industry partners. Financial support was also provided by the University of Melbourne postgraduate scholarship scheme.