Journal article
Genome-wide association studies of 74 plasma metabolites of German shepherd dogs reveal two metabolites associated with genes encoding their enzymes
PXY Soh, JM Marin Cely, SA Mortlock, CJ Jara, R Booth, S Natera, U Roessner, B Crossett, S Cordwell, M Singh Khatkar, P Williamson
Metabolomics | SPRINGER | Published : 2019
Abstract
Introduction: German shepherd dogs (GSDs) are a popular breed affected by numerous disorders. Few studies have explored genetic variations that influence canine blood metabolite levels. Objectives: To investigate genetic variants affecting the natural metabolite variation in GSDs. Methods: A total of 82 healthy GSDs were genotyped on the Illumina CanineHD Beadchip, assaying 173,650 markers. For each dog, 74 metabolites were measured through liquid and gas chromatography mass spectrometry (LC–MS and GC–MS) and were used as phenotypes for genome-wide association analyses (GWAS). Sliding window and homozygosity analyses were conducted to fine-map regions of interest, and to identify haplotypes ..
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Funding Acknowledgements
This work was supported by the Canine Research Foundation. This research is supported by an Australian Government Research Training Program (RTP) Scholarship. Metabolites were extracted and analysed from plasma at Metabolomics Australia (School of BioSciences, University of Melbourne, Australia), a National Collaborative Research Infrastructure Strategy (NCRIS) initiative under Bioplatforms Australia, Pty Ltd. The authors would like to thank Himasha Mendis, Nirupama Jayasinghe and Alice Ng from Metabolomics Australia who extracted and analysed metabolites. The authors would also like to thank the owners and dogs that donated samples for this study.