A rapid and inexpensive viability assay for zoospores and zoosporangia of Batrachochytrium dendrobatidis
Rebecca Jane Webb, Lee Berger, Lee F Skerratt, Alexandra A Roberts
Journal of Microbiological Methods | ELSEVIER | Published : 2019
The fungus Batrachochytrium dendrobatidis is causing global amphibian declines. Here we describe a simple, rapid and inexpensive methylene blue staining protocol to determine B. dendrobatidis viability, regardless of life-stage. The viability of cells in suspension or adherent monolayers can be determined using either manual microscopy counting or colorimetric assay.
Awarded by Morris Animal Foundation Pilot Grant
This work was supported by the Morris Animal Foundation Pilot Grant D18ZO-852 and the Holsworth Wildlife Research Endowment.