Journal article

Chemical Modification of Cellulose Membranes for SPOT Synthesis

Wenyi Li, John Wade, Eric Reynolds, Neil M O'Brien-Simpson



Since the development of solid-phase peptide synthesis in the 1960s, many laboratories have modified the technology for the production of peptide arrays to facilitate the discovery of novel peptide mimetics and therapeutics. One of these, known as SPOT synthesis, enables parallel peptide synthesis on cellulose paper sheets and has several advantages over other peptide arrays methods. Today, the SPOT technique remains one of the most frequently used methods for synthesis and screening of peptides on arrays. Although polypropylene and glass can be used for the preparation of peptide arrays, the most commonly used material for SPOT membranes is cellulose. Critical to the success of the SPOT syn..

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Awarded by NHMRC

Funding Acknowledgements

The National Health and Medical Research Council (NHMRC) of Australia and Australian Research Council (ARC) are thanked for financial support over many years for the peptide chemistry and chemical biology studies reported in the authors' laboratories. WL is the recipient of the Weary Dunlop Foundation 2019 Grant for the development of antimicrobial peptides. JDW is an NHMRC Principal Research Fellow (grant numbers APP628404 and 1117483). NMOS is the recipient of NHMRC funding (grant numbers APP1142472, APP1158841) and Australian Dental Research Funding in antimicrobial materials. Research at The Florey Institute of Neuroscience and Mental Health is supported by the Victorian Government Operational Infrastructure Support Program. We thank Professor Frances Separovic for many years of outstanding collaboration and friendship and wish her well in her retirement.