Journal article

Quantification of T-Cell and B-Cell Replication History in Aging, Immunodeficiency, and Newborn Screening

Ruud HJ Verstegen, Pei M Aui, Eliza Watson, Samuel De Jong, Sophinus JW Bartol, Julian J Bosco, Paul U Cameron, Robert G Stirling, Esther de Vries, Jacques JM van Dongen, Menno C van Zelm

FRONTIERS IN IMMUNOLOGY | FRONTIERS MEDIA SA | Published : 2019

Abstract

Quantification of T-cell receptor excision circles (TRECs) has impacted on human T-cell research, but interpretations on T-cell replication have been limited due to the lack of a genomic coding joint. We here overcome this limitation with multiplex TRG rearrangement quantification (detecting ~0.98 alleles per TCRαβ+ T cell) and the HSB-2 cell line with a retrovirally introduced TREC construct. We uncovered 10 cell divisions in effector memory T-cell subsets. Furthermore, we show that TREC dilution with age in healthy adults results mainly from increased T cell replication history. This proliferation was significantly increased in patients with predominantly antibody deficiency. Finally, Guth..

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University of Melbourne Researchers