Human Endometrial Extracellular Vesicles Functionally Prepare Human Trophectoderm Model for Implantation: Understanding Bidirectional Maternal-Embryo Communication
Jemma Evans, Alin Rai, Hong PT Nguyen, Qi Hui Poh, Kirstin Elglass, Richard J Simpson, Lois A Salamonsen, David W Greening
Proteomics | WILEY | Published : 2019
Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial-embryo cross talk and facilitate implantation remain largely unknown. Extracellular vesicles (EVs) are emerging as important mediators of this process. Here, a trophectoderm spheroid-based in vitro model mimicking the pre-implantation human embryo is used to recapitulate important functional aspects of blastocyst implantation. Functionally, human endometrial EVs, derived from hormonally treated cells synchronous with implantation, are readily internalized by trophectoderm ce..View full abstract
Awarded by NHMRC Project
J.E., H.N., L.A., and D.G conceived and designed the experiments. J.E., H.N., and D.G. carried out the majority of experiments. J.E. performed the TSC spheroid generation and functional experiments. K.A. performed the confocal microscopy studies. A.R. performed informatics and data interrogation. All authors analyzed the data. J.E., A.R., L.A., and D.G. wrote, reviewed, and edited the manuscript. All authors approved the final manuscript. This work was supported by NHMRC Project grants #1057741 and #1139489, and by the Victorian Government's Operational Infrastructure funding to the Hudson Institute. D.W.G. was supported by La Trobe Institute for Molecular Science Fellowship and La Trobe University Leadership RFA grant. J.E. was supported by a Fielding Fellowship from the Hudson Institute of Medical Research. TSC cells were generously provided by Professor Susan Fisher, University of California, San Francisco, CA, USA.