VARIATION IN OUTER-MEMBRANE PROTEIN AND LIPOPOLYSACCHARIDE PROFILES OF PASTEURELLA-HAEMOLYTICA ISOLATES OF SEROTYPES A1 AND A2 OBTAINED FROM PNEUMONIC AND HEALTHY CATTLE

Abstract

The outer-membrane protein (OMP) and lipopolysaccharide (LPS) profiles of 29 isolates of Pasteurella haemolytica serotypes A1 (18 isolates) and A2 (11 isolates), obtained from pneumonic (13 isolates) or healthy (16 isolates) cattle, were compared by SDS-PAGE and Western blot analysis. Coomassie-blue-stained OMP profiles of serotype A1 and A2 isolates could be distinguished from each other by differences in both major and minor proteins. Whereas the OMP profiles of the serotype A1 isolates were extremely uniform in stained gels, there was variation in the mobilities of high-molecular-mass minor proteins and one of the major proteins of serotype A2 isolates. Differences in the OMP profiles of ..