Journal article

Mitochondrial Fusion by M1 Promotes Embryoid Body Cardiac Differentiation of Human Pluripotent Stem Cells

Jarmon G Lees, Anne M Kong, Yi C Chen, Priyadharshini Sivakumaran, Damian Hernandez, Alice Pebay, Alexandra J Harvey, David K Gardner, Shiang Y Lim

STEM CELLS INTERNATIONAL | HINDAWI LTD | Published : 2019

Abstract

Human induced pluripotent stem cells (iPSCs) can be differentiated in vitro into bona fide cardiomyocytes for disease modelling and personalized medicine. Mitochondrial morphology and metabolism change dramatically as iPSCs differentiate into mesodermal cardiac lineages. Inhibiting mitochondrial fission has been shown to promote cardiac differentiation of iPSCs. However, the effect of hydrazone M1, a small molecule that promotes mitochondrial fusion, on cardiac mesodermal commitment of human iPSCs is unknown. Here, we demonstrate that treatment with M1 promoted mitochondrial fusion in human iPSCs. Treatment of iPSCs with M1 during embryoid body formation significantly increased the percentag..

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Grants

Awarded by Australian Research Council Future Fellowship


Awarded by NHMRC Senior Research Fellowship


Funding Acknowledgements

This work was carried out with support from the St. Vincent's Hospital (Melbourne) Research Endowment Fund and Stafford Fox Medical Research Foundation. AP is supported by an Australian Research Council Future Fellowship (FT140100047) and a NHMRC Senior Research Fellowship (APP1136023). The O'Brien Institute Department of St. Vincent's Institute of Medical Research and the Centre for Eye Research Australia receive Operational Infrastructure Support from the Victorian State Government's Department of Innovation, Industry and Regional Development. We thank James A. Thomson (University of Wisconsin) for providing the iPS-Foreskin-2 cell line.