A global genotyping survey of Strongyloides stercoralis and Strongyloides fuelleborni using deep amplicon sequencing
Joel LN Barratt, Meredith Lane, Emir Talundzic, Travis Richins, Gemma Robertson, Fabio Formenti, Bobbi Pritt, Guilherme Verocai, Joelma Nascimento de Souza, Neci Mato Soares, Rebecca Traub, Dora Buonfrate, Richard S Bradbury
PLoS Neglected Tropical Diseases | PUBLIC LIBRARY SCIENCE | Published : 2019
Strongyloidiasis is a neglected tropical disease caused by the human infective nematodes Strongyloides stercoralis, Strongyloides fuelleborni fuelleborni and Strongyloides fuelleborni kellyi. Previous large-scale studies exploring the genetic diversity of this important genus have focused on Southeast Asia, with a small number of isolates from the USA, Switzerland, Australia and several African countries having been genotyped. Consequently, little is known about the global distribution of geographic sub-variants of these nematodes and the genetic diversity that exists within the genus Strongyloides generally. We extracted DNA from human, dog and primate feces containing Strongyloides, collec..View full abstract
Awarded by CDC Office of Advanced Molecular Detection (AMD)
Awarded by Italian Ministry of Health
This study was supported by the CDC Office of Advanced Molecular Detection (AMD) grant AMD133 - Advanced Molecular Detection of Parasitic Pathogens - (https://www.cdc.gov/ncezid/who-we-are/ncezid-divisions/oamd.html). Funding for the collection and identification of some specimens employed was provided by the Italian Ministry of Health "Fondi Ricerca Corrente - Linea 2, progetto 6" to IRCCS Sacro Cuore Don Calabria Hospital. DB - Linea 2, progetto 6" - The Italian Ministry of Health "Fondi Ricerca Corrente" (http://www.salute.gov.it/portale/temi/p2_5.jsp?lingua=italiano&area=Ricerca%20sanitaria&menu=corrente). The sponsors or funders did not play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.