Journal article
Comparison of beaming and droplet digital PCR for circulating tumor DNA analysis
B O’Leary, S Hrebien, M Beaney, C Fribbens, I Garcia-Murillas, J Jiang, Y Li, CH Bartlett, F André, S Loibl, S Loi, M Cristofanilli, NC Turner
Clinical Chemistry | AMER ASSOC CLINICAL CHEMISTRY | Published : 2019
Abstract
BACKGROUND: Circulating tumor DNA (ctDNA) assays are increasingly used for clinical decision-making, but it is unknown how well different assays agree. We aimed to assess the agreement in ctDNA mutation calling between BEAMing (beads, emulsion, amplification, and magnetics) and droplet digital PCR (ddPCR), 2 of the most commonly used digital PCR techniques for detecting mutations in ctDNA. METHODS: Baseline plasma samples from patients with advanced breast cancer enrolled in the phase 3 PALOMA-3 trial were assessed for ESR1 and PIK3CA mutations in ctDNA with both BEAMing and ddPCR. Concordance between the 2 approaches was assessed, with exploratory analyses to estimate the importance of samp..
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Awarded by Merck
Funding Acknowledgements
The authors thank Breast Cancer Now for funding this work as part of Program Funding to the Breast Cancer Now Toby Robins Research Centre.