Journal article
The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology
TE Wood, SA Howard, A Förster, LM Nolan, E Manoli, NP Bullen, HCL Yau, A Hachani, RD Hayward, JC Whitney, W Vollmer, PS Freemont, A Filloux
Cell Reports | CELL PRESS | Published : 2019
Open access
Abstract
The bacterial type VI secretion system (T6SS) delivers effector proteins into prokaryotic and eukaryotic cells to enhance the survival of the donor cell. Wood et al. describe an antibacterial T6SS toxin family eliciting a profound cell division defect and lysis. The structure of this periplasmic-acting toxin reveals a metallopeptidase fold.
Grants
Awarded by Imperial College London
Funding Acknowledgements
The authors are grateful to Luke P. Allsopp, Sarah Wettstadt, and Silvia D'Arcangelo for their technical help; Serge Mostowy for microscopy support; Thibaut Leger and Camille Garcia at the Institut Jacques Monod proteomic facility, UMR7592, Universite Paris Diderot/CNRS, for mass spectrometry analysis; Despoina A.I. Mavridou and R. Christopher D. Furniss (CMBI, Imperial College London) for E. coli MC1000 strains; Suzana P. Salcedo (IBCP, Lyon) for A. baylyi ADP1; and Gad Frankel (CMBI, Imperial College London) for S. arizonae genomic DNA. T.E.W. and S.A.H.are in receipt of PhD scholarships from the Wellcome Trust and Medical Research Council (MRC), respectively. E.M. is supported by MRC grant MR/N023250/1; L.M.N. is supported by MRC grant MR/N023250/1 and a Marie Curie Fellowship (PIIF-GA-2013-625318); N.P.B. is supported by a Canada Graduate Scholarship; H.C.L.Y. and W.V. are supported by Wellcome Trust grant 101824/Z/13/Z; A.H. is supported by H2020-MSCA-Global Fellowship grant 657766; R.D.H. is supported by MRC grant N000846/1; J.C.W. is supported by Canadian Institutes of Health Research (CIHR) grant PJT-156129; P.S.F. and A. Forster are supported by MRC grant MRK/K001930/1; and A. Filloux is supported by MRC grants MR/N023250/1 and MRK/K001930/1.