Spatiotemporal Expression of 3-B-3(-) and 7-D-4 Chondroitin Sulfation, Tissue Remodeling, and Attempted Repair in an Ovine Model of Intervertebral Disc Degeneration.

Brooke Farrugia; Susan M Smith; Cindy C Shu; James Melrose

Journal article

Spatiotemporal Expression of 3-B-3(-) and 7-D-4 Chondroitin Sulfation, Tissue Remodeling, and Attempted Repair in an Ovine Model of Intervertebral Disc Degeneration.

Brooke Farrugia, Susan M Smith, Cindy C Shu, James Melrose

Cartilage | SAGE Publications | Published : 2020

DOI: 10.1177/1947603519876354

Abstract

OBJECTIVE: Examination of intervertebral disc (IVD) regeneration in an ovine annular lesion model. HYPOTHESIS: Sulfation motifs are important functional determinants in glycosaminoglycans (GAGs). Previous studies have correlated 3-B-3(-) and 7-D-4 chondroitin sulfate (CS) motifs in tissues undergoing morphogenetic transition in development. We hypothesize that these motifs may also be expressed in degenerate IVDs and may represent a reparative response. DESIGN: Induction of disc degeneration by 5 mm or 6 × 20 mm lesions in the annulus fibrosus (AF) over 6 or 3 to 6 months postoperation (PO). Tissue sections were stained with toluidine blue-fast green, 3-B-3(-) and 7-D-4 CS-sulfation motifs w..

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University of Melbourne Researchers

Brooke Farrugia Author

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

Profs. Andrew and Christine Dart (Sydney University Veterinary Hospital) and Prof. Christopher B. Little (Institute of Bone and Joint Research, University of Sydney) are thanked for undertaking the sheep surgeries used in this study. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was funded by NHMRC Project Grant 1004032.