Genome-Wide Screen for New Components of the Drosophila melanogaster Torso Receptor Tyrosine Kinase Pathway
Alex R Johns, Michelle A Henstridge, Melissa J Saligari, Karyn A Moore, James C Whisstock, Coral G Warr, Travis K Johnson
G3-GENES GENOMES GENETICS | GENETICS SOCIETY AMERICA | Published : 2018
Patterning of the Drosophila embryonic termini by the Torso (Tor) receptor pathway has long served as a valuable paradigm for understanding how receptor tyrosine kinase signaling is controlled. However, the mechanisms that underpin the control of Tor signaling remain to be fully understood. In particular, it is unclear how the Perforin-like protein Torso-like (Tsl) localizes Tor activity to the embryonic termini. To shed light on this, together with other aspects of Tor pathway function, we conducted a genome-wide screen to identify new pathway components that operate downstream of Tsl. Using a set of molecularly defined chromosomal deficiencies, we screened for suppressors of ligand-depende..View full abstract
We thank James Heaney, the Australian Drosophila Biomedical Research Facility (OzDros), and Monash Micro Imaging for technical support. We thank the Bloomington and Kyoto Drosophila Stock Centers and David Hipfner for providing fly stocks. M.A.H. is a National Health and Medical Research Council (NHMRC) of Australia Early Career Fellow. T.K.J. is an Australian Research Council (ARC) Discovery Early Career Researcher Award Fellow. J.C.W. is a NHMRC Senior Principal Research Fellow, and he further acknowledges the support of an ARC Federation Fellowship. This project was supported by an ARC grant to J.C.W. and C.G.W., and used resources developed under a Monash University Interdisciplinary Research Grant award.