Fate of Antibiotic Resistant Pseudomonas putida and Broad Host Range Plasmid in Natural Soil Microcosms
Xiao-Ting Fan, Hu Li, Qing-Lin Chen, Yu-Sen Zhang, Jun Ye, Yong-Guan Zhu, Jian-Qiang Su
Frontiers in Microbiology | FRONTIERS MEDIA SA | Published : 2019
Plasmid conjugation is one of the dominant mechanisms of horizontal gene transfer, playing a noticeable role in the rapid spread of antibiotic resistance genes (ARGs). Broad host range plasmids are known to transfer to diverse bacteria in extracted soil bacterial communities when evaluated by filter mating incubation. However, the persistence and dissemination of broad range plasmid in natural soil has not been well studied. In this study, Pseudomonas putida with a conjugative antibiotic resistance plasmid RP4 was inoculated into a soil microcosm, the fate and persistence of P. putida and RP4 were monitored by quantitative PCR. The concentrations of P. putida and RP4 both rapidly decreased w..View full abstract
Awarded by National Key Research and Development Program from Ministry of Science and Technology
Awarded by National Natural Science Foundation of China
This study was financially supported by the National Key Research and Development Program from Ministry of Science and Technology (2016YFD0800205), National Natural Science Foundation of China (31722004), K.C. Wong Education Foundation and Youth Innovation Promotion Association, CAS.