Monoclonal Antibody Productivity of Hybridoma Cells Under Active Hypothermic Growth Condition

Abstract

In our previous study, the enhanced specific monoclonal antibody (mAb) productivity of hybridoma cells grown under mild hypothermic condition (32 °C) was proportional to the population of S-phase cells, and inversely proportional to the population of G1-phase cells. However, the volumetric mAb production at 32 °C was lower compared to 37 °C due to its lower maximum viable cell density. This finding implies that control proliferation at G1 phase is not the priority factor in determining the productivity of mAb under hypothermic condition. Hence, in the present study, the effect of active hypothermic growth on the monoclonal antibody productivity of hybridoma C2E7 was investigated. The growth ..