Journal article

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, Sabine Adam-Klages, William W Agace, Nima Aghaeepour, Muebeccel Akdis, Matthieu Allez, Larissa Nogueira Almeida, Giorgia Alvisi, Graham Anderson, Immanuel Andrae, Francesco Annunziato, Achille Anselmo, Petra Bacher, Cosima T Baldari, Sudipto Bari, Vincenzo Barnaba Show all

European Journal of Immunology | WILEY | Published : 2019

Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-revi..

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Grants

Awarded by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)


Awarded by Netherlands Organisation for Scientific Research - Domain Applied and Engineering Sciences (NWO-TTW), research program VENI


Awarded by DFG


Awarded by Excellence Cluster "Inflammation at Interfaces"


Awarded by German Research Foundation


Awarded by NIH


Awarded by Deutsche Forschungsgemeinschaft


Awarded by Germany Research Foundation


Awarded by European Union Innovative Medicines Initiative - Joint Undertaking


Awarded by Else Kroner-Fresenius-Foundation, German Federal Ministry of Education and Research e:Med sysINFLAME Program


Awarded by European Research Council under the European Union


Funding Acknowledgements

Work in the laboratory of Dieter Adam is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)-Projektnummer 125440785 - SFB 877, Project B2.