Journal article

Modes of carbon dioxide delivery during laparoscopy generate distinct differences in peritoneal damage and hypoxia in a porcine model

Shienny Sampurno, Timothy J Chittleborough, Sandra Carpinteri, Jonathan Hiller, Alexander Heriot, Andrew Craig Lynch, Robert George Ramsay



BACKGROUND: Insufflation with CO2 can employ continuous flow, recirculated gas and/or additional warming and humidification. The ability to compare these modes of delivery depends upon the assays employed and opportunities to minimize subject variation. The use of pigs to train colorectal surgeons provided an opportunity to compare three modes of CO2 delivery under controlled circumstances. METHODS: Sixteen pigs were subjected to rectal resection, insufflated with dry-cold CO2 (DC-CO2) (n = 5), recirculated CO2 by an AirSeal device (n = 5) and humidification and warming (HW-CO2) by a HumiGard device (n = 6). Peritoneal biopsies were harvested from the same region of the peritoneum for fixati..

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Funding Acknowledgements

This work was supported in part by the Epworth Hospital Research Foundation (Drs Lynch and Ramsay) and the National Health and Medical Research Council of Australia (Drs Heriot and Ramsay). Fisher and Paykel Healthcare made a loan of HumiGards for this study. Ms Sampurno, Drs Chittleborough, Carpinteri and Hiller and Heriot have no disclosures. Drs Lynch and Ramsay have received research grant funding from Fisher and Paykel Healthcare for separate small animal studies. Prof Ramsay also receives research support associated with unconnected clinical trials from Targovax, Oslo; Merck Serono, Germany and Beigene Sciences, Australia.